Generator

Part:BBa_J100122

Designed by: Malcolm Campbell   Group: Campbell M Lab   (2013-09-06)

P5+riboswitch+adhE

This is a strong promoter driving transcription of theophylline riboswitch D followed by E. coli optimized adhE gene. Used in combination with adhE deletion strain SHH31. Deletion of a Valine codon between bp 176 and 177.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 649
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 101
    Illegal AgeI site found at 734
    Illegal AgeI site found at 1958
    Illegal AgeI site found at 2003
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None